Electrophoretic Profiles of the Outer Membrane Proteins of Pasteurella Multocida Strains Isolated from Cattle

Forty Pasteurella multocida strains of serotype B:2,5 were collected from cattle affected with haemorrhagic septicaemia, and 72 strains mainly of serotype A:3, were isolated from calves with changes typical of bronchopneumonia. The outer membrane proteins (OMPs) were prepared by extraction, with 1% sarcosyl from field strains and two reference strains. OMPs were separated electrophoretically at a 10% gradient on polyacrylamide gel in SDS-PAGE. The densitograms of the examined strains were compared. The densitometric analysis showed some qualitative and quantitative differences in the protein electrophoretic profiles of the isolates. The most important differences in molecular weights and optical density of protein bands were noted in the 30 kDa to 38 kDa range. Strains isolated from adult cattle and calves were classified into two and four OMPs types, respectively.